Cost Anaylsis
Nucleic Acid Extraction
Example of Reagents used for Lysis Buffer
| Reagent | Concentration | Volume/Amount | Cost | Total Cost ($) | Link |
|---|---|---|---|---|---|
| Tris - HCl | 400 mM | 63.04 g | $ 0.352 / g | 22.19 | https://www.sigmaaldrich.com/catalog/product/sigma/t5941?lang=en®ion=AE |
| EDTA | 60 mM | 17.53 g | $0.18 / g | 3.16 | https://www.sigmaaldrich.com/catalog/product/sial/eds?lang=en®ion=AE |
| NaCl | 150 mM | 8.77 g | $0.04121 / g | 0.36 | https://www.sigmaaldrich.com/catalog/product/sigald/s9888?lang=en®ion=AE |
| SDS | 1% w/v | 10 g | $0.577 / g | 5.77 | https://www.sigmaaldrich.com/catalog/product/sigma/l3771?lang=en®ion=AE |
| pH | 8.0 | ||||
| Untitled |
Note for table above: This is assuming 1 L of lysis buffer is prepared.^
Grand Total: $31.48 for 1 L
Lysing Cost Per Diagnostic Test: $0.01574
^ Assuming 500 μl of lysis buffer is needed per diagnostic test.
Nucleic acid detection
- RPA reaction
Reagents used
| Reagent | Cost | Cost per test ($) | Link |
|---|---|---|---|
| Primers | 0.093 | ||
| TwistAmp® Basic kit (lyophilized enzyme + rehydration buffer + Magnesium acetate) | £240/ 96 reactions (lyophilized mix) & 1 lyophilized mix covers 3 rpa reactions | 1.09 | https://www.twistdx.co.uk/en/products/product/twistamp-basic |
| Untitled |
2. LAMP reaction
Reagents used
| Reagent | Cost | Cost per test ($) | Link |
|---|---|---|---|
| Primers | 0.093 | ||
| Isothermal Mastermix | $1322 /300 reactions | 4.40 |
3. CRISPR-Cas12 reaction
Reagents used
| Reagent | Cost | Cost per test ($) | Link |
|---|---|---|---|
| Cas enzyme | 0.375 | ||
| NEBuffer | 0.0108 | ||
| gRNA | 0.136 | ||
| REPORTER (UNDECIDED) | |||
| * option1: FQ quencher | 0.24 |
Current Method
- Nucleic Acid extraction
Kit/ Reagent used
| Reagents | Details | Cost | Cost per test ($) | Link |
|---|---|---|---|---|
| DNeasy Blood & Tissue Kits | $171.00/ 50 reactions | $3.42 | https://www.qiagen.com/us/products/top-sellers/dneasy-blood-and-tissue-kit/#orderinginformation | |
| DNeasy PowerClean CleanUp Kit Print | $228.00/ 50 reactions | $4.56 | https://www.qiagen.com/se/products/new-products/dneasy-powerclean-cleanup-kit/#orderinginformation |
2. Nucleic Acid Detection
Kit/ Reagent used
| Reagents | Details | Cost | Cost per test ($) | Link |
|---|---|---|---|---|
| Techne™ PrimePRO QPCR DNA detection Kit, Batrachochytrium dendrobatidis (with Mastermix) | Lyophilized primer and probe mix, Lyophilized master mix, Lyophilized master mix resuspension buffer, Lyophilized ROX Ampule, High-copy DNA for standard curve, Internal extraction control DNA, Beta-actin endogenous control, RNAse/DNAse free water | £742.89 / 150 reactions | $6.46 | https://www.fishersci.co.uk/shop/products/techne-primepro-qpcr-dna-detection-kit-batrachochytrium-dendrobatidis-with-mastermix/15371278http://www.techne.com/docs/b_dendrobatidis.pdf |